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KMID : 0613820200300050428
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Journal of Life Science
2020 Volume.30 No. 5 p.428 ~ p.433
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Pupal Drone Extracts for Anti-wrinkle and Skin-lightening Materials
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Kim Jung-Eun
Kim Do-Ik
Koo Hui-Yeon
Kim Hyeon-Jin
Kim Seong-Yeon
Lee Yoo-Beom
Moon Jae-Hak
Choi Yong-Soo
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Abstract
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In this study, we created pupal stage extracts of Apis mellifera L. drones for use in cosmetic materials. The effect of the drone pupae extract (DPE) on HDF cells was assessed for analysis of anti-wrinkle activity by collagen or collagenase gene expression, and the skin-lightening effect was studied by in vitro tyrosinase inhibition and B16F10 melanoma assay; the two cells were found to be non-cellular when the concentration of DPE was 100 μg/ml. Albutin concentration (positive control) in the whitening test was set at a capacity of 100 ug/ml and m-melanocyte stimulating hormone (α-MSH). A melanin-producing induction material was set at a concentration of 100 nM, and the expression of collagen type I and MMP1 collagenase was measured using HDF cells. MMP1 expression was seen to reduce in a concentration-dependent manner in treatment with DPE. Inhibiting melanin generation with B16F12 cells indicated a tendency to decrease in the DPE treatment group. Both L-Tyrosine and L-DOPA as DPE were used in an in vitro tyrosinase induction test to demonstrate the effects of tyrosinase suppression on concentrations. The higher the concentration of DPE, the greater the wrinkle reduction and whitening effect. In conclusion, it was found that DPE is an effective smoothing and whitening material by increasing collagen generation and inhibiting collagenase expression and reducing melanin production.
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KEYWORD
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Anti-wrinkles, drone, drone pupa extract, honey bee, skin-lighting
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